Structure-based rational design of a phosphotriesterase.
نویسندگان
چکیده
In silico substrate docking of both stereoisomers of the pesticide chlorfenvinphos (CVP) in the phosphotriesterase from Agrobacterium radiobacter identified two residues (F131 and W132) that prevent productive substrate binding and cause stereospecificity. A variant (W131H/F132A) was designed that exhibited ca. 480-fold and 8-fold increases in the rate of Z-CVP and E-CVP hydrolysis, respectively, eliminating stereospecificity.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 75 15 شماره
صفحات -
تاریخ انتشار 2009